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CURRICULUM VITAE

Robert Wallace Malone, MD, MS

MEDICAL LICENSURE: Physician and Surgeon, State of Maryland

 

CONTACT: 771 Mitchell Rd, Jasper, GA 30143 robert@cieloazure.com

EDUCATION: (Education funded via scholarships, self -part-time work, loans)

  • Post graduate Fellowship award Bank of America-Giannini Found. Medical Research. (6/92 to 6/93)
  • University of California, Davis, Medical Center. Clinical Pathology Internship. June 1992
  • Northwestern University Medical School. Doctor of Medicine. June 1991
  • University of California, San Diego. Master of Science, Biology. December 1988
  • University of California, Davis. Bachelor of Science, Biochemistry. June 1984
  • Santa Barbara City College. Santa Barbara, California. Associate of Arts, General Studies/computer sciences. June 1982

EMPLOYMENT:

Solvay Pharmaceuticals, Director. Nov 2006- present

Summitt Drug Development Services, Senior Medical Director 10/05-11/06

Aeras Global TB Vaccine Foundation. Director, Business Development and Project Management 8/04-6/05

Gene Delivery Alliance, Inc. President and founder. Consulting, research, development and intellectual property licensing firm. 4/2001-present

  • Consulting services for IOMAI cooperation, proposal development and Clinical Vaccine trial expertise.
  • Consulting services for Aeras Global Tuberculosis Vaccine Foundation. Proposal development and intellectual property assistance.
  • Consulting services for VaxGen, Inc. Proposal development relating to bioterrorism countermeasures and clinical trials.
  • Consulting services for ID Biomedical, Inc. Preparation of regulatory documents relating to clinical vaccine trials.
  • Consulting services for MSD, Inc., Business and technology development planning. Title: senior consultant at MSD
  • Consulting services for Inovio, AS. Business and technology development planning. Intellectual property development
  • Intellectual property development and research at the University of Bern, Switzerland.
  • Consulting services for Molecular Histology, Inc. Title: Medical Director

Aeras Global TB Vaccine Foundation. Director, Business Development and Project Management. 8/2004 - 5/2005

Associate Director, Clinical Research. Dynport Vaccine Company, LLC

  • 10/2002-10/2003
  • Dynport vaccine company (www.dynport.com) is a government contractor that holds the primary contract for advanced development of biodefense vaccines for the US Department of Defense.
  • Serving as liaison between product development teams and clinical research support group
  • Preparation of planning documents and product development plans
  • Identification of new technologies relevant to product development teams, facilitating integration of same in product development plans
  • Preparing documents for clinical trials including investigator brochures
  • Preparing proposal solicitations, technical review of subcontractor proposals
  • Serving as senior staff for technology watch program
  • Technical review of potential subcontractors, new technologies
  • Preparation of technical reviews for monthly in-house publication
  • Assisting business development group in strategic evaluation and planning concerning new business opportunities

Co-founder, CSO, Board of Director Member

Intradigm, Corp. 1999 (incorp date: 2/2000) to 2001.

  • Intradigm is a small biotech start up focused on target validation using non-viral gene delivery technology. The core scientific concepts were initially developed in Dr. Malone's laboratory, both iscientific concepts were initially developed in Dr. Malone's laboratory, both independently and in collaboration with Regeneron, Inc.
  • One of three co-founders
  • Helped to secure 2.3 million in V.C. funding, including monies from the Novartis Venture Fund, ETP Venture Capital Fund and the State of Maryland.
  • Facilities set-up, infrastructure set-up
  • Business and technology development planning, including in-depth business and Scientific plan.
  • Intellectual Property Development

Assoc Professor of Surgery (adjunct)

  • USUHS Uniformed Services University Health Sciences, 3/2001-Present.

Adjunct Professor (adjunct)

  • University of South Florida, Dept of Chemical Engineering, 8/2000-present.

Chief of Laboratory Science, and Director of Tissue Banking

  • Dept of Surgery, USUHS, Clinical Breast Care Program (CBCP) through the Henry M. Jackson Foundation (Aug. 2000 to April 2001)
  • Set-up laboratory facilities for the Clinical Breast Care Project, including new facilities design (tissue banking facilities, laboratory, animal rooms, animal surgical suite, office suites) at USUHS and Windber Medical Center, PA.
  • Hired faculty, technicians, staff for CBCP at both sites, including writing and initiating job descriptions, job interviews, hiring decisions, set-up for re-locations.
  • Laboratory Supervisor: Tissue banking immunology, cell culture, gene transfer, genetic vaccination research, animal research.
  • Worked closely with architect firm to design space.
  • Set-up equipment and laboratory purchases, including vendor price quotes, equipment specs.
  • Wrote initial budgets and supervised equipment purchases.
  • Wrote animal protocols and grants.

Assistant Professor

  • University of Maryland, Baltimore School of Medicine, Dept. of Pathology (Jan 1997-Aug 2000)
  • Successful grant writing, IACUC protocol writing, budget writing and implementation
  • Set-up and ran successful research laboratory in immunology (genetic vaccination), gene transfer (pulmonary, dermal, heart, liver). See papers/abstract/presentations for more detail.
  • Laboratory set-up and employee hiring/training
  • Training of Graduate students, on selection committee for pathology graduate students
  • Teaching responsibilities (see below).
  • Set-up good laboratory safety practices.
  • Member, Oncology Program, University of Maryland Greenebaum Cancer Center
  • Member, Molecular and Cell Biology Graduate Division, University of Maryland, Baltimore
  • Member, Comparative Medicine Program, University of Maryland, Baltimore

Assistant Professor

  • University of California, Davis Department of Medical Pathology,
  • Director and Founder, Gene Therapy Prog (October 1993 to January 1997).
  • Successful grant writing, IACUC protocol writing, budget writing and implementation
  • Wrote a DOD genetic vaccination grant that totaled over 2 million in monies for UC Davis
  • Set-up and ran successful research laboratory in immunology (genetic vaccination), gene transfer (pulmonary, dermal, heart, liver). See papers/abstract/presentations for more details..
  • Laboratory set-up and employee hiring/training
  • Training of Graduate students
  • Teaching responsibilities (see below).
  • Set-up good laboratory safety practices.
  • Member, Biochemistry and Molecular Biology Graduate Group
  • Collaborative Investigator, California Regional Primate Research Unit.

Research Fellow

  • Medical Pathology, UC Davis Medical Center. (July 1992 to September 1993)
  • Succesful grant writing
  • Research in genetic (DNA) vaccination and gene transfer. See below for more detail.
  • laboratory set-up

Pathology Resident

  • Medical Pathology, UC Davis Medical Center. (July 1991 to July 1992)
  • Successfully completed First year Internship in pathology

Research Scientist

  • Vical Inc. (January 1989 to September 1989)
  • Set up Vical's molecular biology laboratory
  • Initiated and carried out esearch in non-viral gene therapy and DNA vaccination
  • Inventor of "naked DNA" gene therapy. See issued patents for details.
  • Inventor of DNA vaccination. See issued patents for details.

ACADEMIC HONORS:

  • Listed in Who's Who in America, 2004 and 2005
  • Trainee Investigator Award, American Federation for Clinical Research (4/93)
  • Bank of America-Giannini Foundation Medical Research. Fellow (6/92 to 6/93)
  • Henry Christian Award for Excellence in Research, American Federation for Clinical Res. (5/92)
  • UCDMC Medical Scholars Grant (7/92 to 7/93)
  • First Place, Northwestern AOA Research Symposium competition for Medical Students (1989)
  • USPHS Pre-Doctoral Fellowship (1986-1988)
  • San Diego Supercomputer Grant for RNA structure modeling (1988)
  • Northwestern University MD../Ph.D. Scholarship (1984-1986)
  • Dean's List, UC. Davis (1982-1984)
  • President's Undergraduate Fellowship Grant for investigation of oncogene expression in breast tumor tissue. UC. Davis (9/83-6/84)
  • Edmonson Summer Fellowship, Department of Pathology, UC. Davis Medical School (1984)
  • Outstanding Student at Graduation, SBCC (1982)
  • Outstanding Computer Science Student, SBCC (1982)
  • First Place (Business Division), statewide competition, Bank of America California Community College Awards Program (1982)
  • Service to the College Award, SBCC (1981 and 1982)

 

MEMBERSHIPS:

  • Gene Therapy/Molecular Biology International Society
  • Member, New York Academy of Sciences
  • Member, Bioelectrochemical Society
  • Member, European Gene Therapy Society
  • Member, AAAS

TEACHING EXPERIENCE:

University of Maryland, Medical School

  • Course Director, Fundamentals of Molecular Biology (Graduate Course, Winter 2000)
  • Host defenses and Infectious Diseases, small group instructor (Year 2 Medical School core curriculum, 1998, 1999)

University of California, Davis.

  • MD 410A/410B. General Systemic Pathology (1992, 1993, 1994, 1995, 1996).
  • PTX 202. Principles of Pharmacology and Toxicology-Lecturer (1995, 1996).
  • BCM 214-414. Molecular Medicine-Lecturer (1995, 1996).
  • IM 295 Cytokines-Lecturer (1996).
  • IDI 280. Molecular Basis of Disease-Lecturer (1996).

University of California, San Diego.

  • Biology 111. Cell Biology (Fall 1988). Teaching Assistant under Dr. M. Montal.
  • Biology 123. Embryology laboratory (Spring 1988). Teaching Assistant under Dr. C.Holt

Santa Barbara City College

  • Computer Laboratory (Spring 1981). Teaching Assistant

OFFICES HELD:

  • IEEE Genomics and Bioinformatics Working Group, Participant (2002)
  • Co-chair, Northern Virginia Technology Council BioMedTech committee (2002- 2003)
  • Scientific Advisory Board:
  • (Intradigm, Corp.-a new start-up from Novartis, Inc.) 2000-2001
  • Scientific Advisory Board and External Portfolio Reviewer:
  • Novartis, Inc. (GTI/Systemix & Pharmacogenetics), 1999-2001
  • University of Maryland , Medical School
  • Pathology Education Policy Committee (1999-2000)
  • UC Davis
  • Education Policy Committee (1996-1/97) Graduate Group in Comparative Pathology.
  • Member, Biochemistry and Molecular Biology Graduate Group (1993-1/97)
  • Member, Comparative Pathology Graduate Group (1995-1/97)

Boehringer Mannheim

  • Scientific Advisory Board (1992-93)

Santa Barbara City College:

  • Associated Student Body, President (1981-1982)
  • Associated Student Body, Vice President (Spring 1981)
  • Computer Science Club, Co-Founder and Vice President (1981-1982)

EDITORIAL BOARDS:

  • Gene Therapy/Molecular Biology International Society (1997-present).

CONSULTANT FOR:

  • MSD, Inc. Senior Consultant 2002
  • Inovio, Inc. 2001
  • Windber Medical and Research Institute, 2000-2001
  • MDS Capital 2000
  • Inex Corp. Evaluation of business plan and scientific reviewer. 2000
  • Gene Therapy Incorporated/Novartis. Consultant and member, Scientific Advisory Board, 1999 - 2001

REVIEWER FOR:

  • Nucleic Acids Research (2001-2002)
  • Molecular Therapy (1999-2001)
  • Journal of Molecular Medicine (2000)
  • Human Gene Therapy (1995-present).
  • Gene Therapy Journal (1997-present).
  • NIH Study Section K01 Breast Cancer Study Section (July 1997).
  • NIDDK Special Emphasis Panel Review Committee for competing continuation Program Project (April 1998 and April 1999)
  • NIAID Study Section "Innovative Grant Program for Approaches in HIV Vaccine Research" June 15-16, 1998

SPECIALITY POST GRADUATE COURSEWORK:

  • Walter Reed healthcare System, Department of Clinical Investigation 2000 Research Course. Certificate of completion
  • USUHS Rodent Handling and Techniques Laboratory. Nov 2000. Testing required and passed.
  • USUHS Animal Protocol Writing Workshop. Oct 2000. Certificate of completion.
  • CIPP 909 Research Ethics, University of Maryland, Baltimore Spring 1999
  • Primate handling course: testing required and passed University of Maryland, Baltimore 1999.
  • Biohazardous training course: testing required and passed University of Maryland, Baltimore 1999.
  • Chemical training course: testing required and passed University of Maryland, Baltimore 1999.
  • Radiation Training. Course: testing required and passed University of Maryland, Baltimore 1999.
  • Computer Programming in C, Anne Arundel Community College, Spring 1999
  • Annual Course in Flow Cytometry, Bowdoin College. June 6-13, 1998
  • "The Magic of Conflict" conflict management workshop, Office of Human Resource Services, University of Maryland, Baltimore. Spring 1998
  • Laboratory animal handling course University of California, Davis, 1994-1997
  • Biohazardous training courses, University of California, Davis, 1994-1997
  • Chemical training courses, University of California, Davis, 1994-1997
  • Radiation Training. Courses-University of California, Davis, 1994-1997

PATENTS ISSUED:

  • Induction of a protective immune response in a mammal by injecting a DNA sequence. Felgner P, Wolff J, Rhodes GH, Malone RW, Carson, DA. US Pat. Ser. No. 6,214,804 issued 4/10/01.
  • DNA vaccines for eliciting a mucosal immune response. Malone, RW. and Malone, JG US Pat. Ser. No. 6,110,898 issued 8/29/00.
  • Formulations and methods for generating active cytofectin: polynucleotide transfection complexes Nantz M, Bennett M, Balasubramaniam RP, Aberle AM, Malone RW. US Pat. Ser. No. 5,925,623 7/20/99
  • Cationic Transport Reagents. Bennett M, Nantz M, and Malone RW. US Pat. Ser. No. 5,892,071 issued 4/06/99
  • Polyfunctional cationic cytofectins, formulations and methods for generating active cytofectin: polynucleotide transfection complexes. Nantz M, Bennett M, Balasubramaniam RP, Aberle AM, Malone RW. US Pat. Ser. No. 5,824,812 issued 10/20/98
  • Cationic Transport Reagents. Bennett M, Nantz M, and Malone RW. US Pat. Ser. No. 5,744,625 issued 4/28/98
  • Generation of antibodies through lipid mediated DNA delivery. Felgner P, Wolff J, Rhodes GH, Malone RW, Carson, DA. US Pat. Ser. No. 5,703,055. issued 12/30/97.
  • Induction of a protective immune response in a mammal by injecting a DNA sequence. Felgner P, Wolff J, Rhodes GH, Malone RW, Carson, DA. US Pat. Ser. No. 5,589,466. issued 12/31/96.
  • Delivery of exogenous DNA sequences in a mammal . Felgner P, Wolff J, Rhodes GH, Malone RW, Carson, DA. US Pat. Ser. No. 5,580,859. issued 12/3/96.
  • Cationic Transport Reagents. Bennett M, Nantz M, and Malone RW. US Pat. Ser. No. 5,527,928. issued 6/18/96
  • Methods of delivering a physiologically active polypeptide to a mammal. Felgner P, Wolff J, Rhodes GH, Malone RW, Carson D. US Pat. Ser. No. 6.413.942 issued 7/2/02
  • Expression of exogenous polynucleotide sequences in a vertebrate, mammal, fish, bird or human. Felgner P, Wolff J, Rhodes GH, Malone RW, Carson D. US Pat. Ser. No. 6,673,776 issued 1/06/04.
  • Generation of an immune response to a pathogen. Felgner P, Wolff J, Rhodes GH, Malone RW, Carson D. US Pat. Ser. No. 6.710,035 issued 3/23/04.
  •  

PATENTS SUBMITTED:

  • DNA vaccines for eliciting a mucosal immune response. Malone, J and Malone, RW.
  • Continuation with amended claims of US Pat. Ser. No. 6,110,898 issued 8/29/00.
  • Compounds and Methods for Enhancing Direct In Vivo Gene Transfer. Malone, JG and Malone RW. Full application filed with US PTO on 8/19/99
  • Method for gene transfection and vaccination through skin using electropermeabilization. Glasspool-Malone J, Drabick, J. and Malone RW Full application filed with US PTO on 3/16/01.
  • Synergism Between Lipidic and Electroporation-Mediated Polynucleotide Delivery. Malone, RW and King, AD. Full Patent filed Sept. 24, 2000
  • Electroporative Delivery of Molecules to Organs. Malone RW, Schmid R, Kubisa B, Uduehi A, Ayuni E, Drabick JJ, Glasspool-Malone J. Provisional patent filed (048227-0109) 8/7/2001.

 

PUBLICATIONS:

Glasspool-Malone J. and Malone RW. Enhancing direct in vivo transfection with nuclease inhibitors and pulsed electrical fields. Methods in Enzymology-Gene Therapy Methods 2002;346:72-91. PMID: 11883098

Glasspool-Malone J, Peter Steenland, Ruth McDonald, Rigoberto Sanchez, Tamara Watts, Joseph Zabner and Malone RW., Marked Enhancement of Macaque Respiratory Tissue Transfection by Aurintricarboxylic Acid. J Gene Med. 2002 May-Jun;4(3):323-2.

Drabick, J.J., Glasspool-Malone J., Somiari S., King A., Malone RW. Increased efficiency of cutaneous transfection and polynucleotide immunization using in-vivo electroporation. Mol Ther 2001 Feb, 3(2). 249 - 255.

Somiari S, Glasspool-Malone J, Drabick JJ, Gilbert RA, Heller R, Jaroszeski MJ, Malone RW.

Theory and in vivo application of electroporative gene delivery. Mol Ther. 2000 Sep;2(3):178-87.

Glasspool-Malone J, Somiari S, Drabick JJ, Malone RW. Efficient nonviral cutaneous transfection. Mol Ther. 2000 Aug;2(2):140-6.

Colosimo A, Goncz KK, Holmes AR, Kunzelmann K, Novelli G, Malone RW, Bennett MJ, Gruenert DC. Transfer and expression of foreign genes in mammalian cells. Biotechniques. 2000 Aug;29(2):314-8, 320-2, 324 passim.

Ahearn A, Malone RW. Models of Cationic Liposome Mediated Transfection. Gene Therapy and Molecular Biology (Dec., 1999) Vol 4

Kisich KO, Malone RW, Feldstein PA, Erickson, KL. Specific inhibition of macrophage TNF-alpha expression by In vivo ribozyme treatment. Journal of Immunology (1999)163(4):2008-16

Glasspool-Malone J, Malone RW, "Marked Enhancement of Direct Respiratory Tissue Transfection by Aurintricarboxylic Acid". Human Gene Therapy (1999) 10:1703-1713.

Berlyn KA, Ponniah S, Stass SA, Malone JG, Hamlin-Green G, Lim JK, Fox M, Tricot G, Alexander RB, Mann DL, and Malone RW. Developing Dendritic Cell Polynucleotide Vaccination for Prostate Cancer Immunotherapy. J. of Biotechnology (1999) 73:155-179.

Bennett MJ, Aberle AM, Balasubramaniam RP, Malone JG, Malone RW, Nantz MH. Cationic lipid mediated gene delivery to murine lung: correlation of polar domain structure with in vivo transfection activity. J. Med. Chem. (1997) 40(25), 4069-4078.

Malone JG, Bergland P, Liljestrom P, Rhodes, GH, Malone RW. Mucosal immune responses associated with polynucleotide vaccination. Behring Inst. Mitt. (1997) 98, 63-72.

Freedland SJ, Malone RW, Borchers HM, Zadourian Z, Malone JG, Bennett MJ, Nantz MH, Juan-Hua L, Gummerlock PH, Erickson KL. Toxicity of cationic lipid-ribozyme in human prostate tumor cells can mimic ribozyme activity. Biochem. and Molecular Medicine. (1996) 59,144-153.

Bennett MJ, Aberle AM, Balasubramaniam RP, Malone JG, Nantz MH, Malone RW. Considerations for the design of improved cationic amphiphile-based transfection reagents. Journal of Liposome Research (1996) 6(3), 545-565.

Aberle AM, Bennett MJ, Malone RW, Nantz MH. The counterion influence on cationic lipid-mediated transfection of plasmid DNA. Biochemica et Biophysica Acta (1996) 1299(3), 281-283.

Balasubramaniam RP, Bennett MJ, Aberle AM, Malone JG, Nantz MH, Malone RW. Structural and functional analysis of cationic transfection lipids: the hydrophobic domain. Gene Therapy (1996) 3(2)163-172.

Montbriand P and Malone RW. An Improved method for the removal of endotoxin from DNA. J Biotechnol. 1996 Jan 26;44(1-3):43-6.

Hickmann MA, Malone RW, Sih TR, Akita GY, Powell JS. Hepatic gene expression after direct DNA injection. Advanced Drug Delivery Reviews (1995) 17(3), 265-271.

Schenborn E, Oler J, Goiffon V, Balasubraniam R, Bennett M, Aberle A, Nantz M, Malone RW.Tfx-50 Reagent, a new transfection reagent for eukaryotic cells. Promega Notes (1995) 52, 2-7.

Bennett MJ, Nantz MH, Balasubraniam RP, Gruenert D, Malone RW. Cholesterol enhances cationic liposome-mediated DNA transfection of human respiratory epithelial cells. Bios Reports (1995) 15, 47-53.

Bennett MJ, Nantz MH, Malone RW. A flexible approach to synthetic lipid ammonium salts for polynucleotide transfection. Tetrahedron Letters (1995) 36(13), 2207-2210.

Malone RW, Hickmann MA, Lehmann-Gruinsma K, Sih TR, Walzem R, Carlson D, Powell JS. Dexamethasone enhancement of gene expression after direct hepatic DNA injection. Journal of Biological Chemistry (1994) 269(47), 29903-29907.

Hickmann MA, Malone RW, Lehmann K, Sih TR, Knoell D, Szoka FC, Walzem R, Carlson DM, Powell JS. Gene expression following direct injection of DNA into liver. Human Gene Therapy (1994) 5, 1477-1483.

Dwarki VJ, Malone RW, Verma IM. Cationic liposome mediated RNA transfection. Methods in Enzymology (1993) 217, 644-654.

Wolff J, Malone RW, Williams P, Chong W, Acsadi G, Jani A, Felgner P. Direct gene transfer into mouse muscle in vivo. Science (1990) 247, 1465-1468.

Malone RW. mRNA Transfection of cultured eukaryotic cells and embryos using cationic liposomes. Focus (1989) 11, 61-66.

Malone RW, Felgner P, Verma I. Cationic Liposome Mediated RNA Transfection. Proceedings of the National Academy of Science USA 86(16), 6077-6081.

Gardner MB, Malone RW, Morris D, Young L, Strange R, Cardiff R, Faulkin L, Mitchell D. Mammary tumors in feral mice lacking MuMTV DNA. Journal of Experimental Pathology 2(2), 93-98.

Faulkin L, Mitchell D, Young L, Morris D, Malone R, Cardiff R, Gardner M. Hyperplastic and neoplastic changes in the mammary glands of feral mice free of endogenous mouse mammary tumor virus provirus. Journal of the National Cancer Institute (1984) 73(4), 971-982.

 

PUBLISHED ABSTRACTS (SELECTED):

 

Effieient rat lung transfection, transplantation and sustained transgene expression by plasmid/ATA electroporation. Schmid RA, Kubisa B, Ayuni E, Cecchini M, Wetterwald A, Glasspool-Malone J, McDonald R, Hyde S, Geiser T, and Malone RW. American Thoracic Society, International Conference. Atlanta, Georgia, USA. May 17-22, 2002 .

Enhancing transfection efficiency in cutaneous wounds using electroporation. Colman K. Byrnes, Robert W. Malone, Suzanne Hilal, Petra H. Nass, Mark D. Duncan, and John W. Harmon. Wound Healing Society and European Tissue Repair Society May 28-June 1, 2002 Baltimore, Maryland, USA May 28-June 1, 2002.

Electroporation greatly enhances intradermal DNA vaccination. Glasspool-Malone J, Drabick JJ, and Malone RW Keystone Sympoisa Conference: Gene-Based Vaccines: Mechanisms, Delivery Systems and Efficacy. Breckenridge, CO April 10-15, 2002.

Imaging Gene Transfer distribution and efficiency in Lung. Cecchini M, Malone RW, Kubisa B, Wetterwald A, McDonald R, Ayuni E, Glasspool-Malone J, Schmid R. Molecular Imaging in Cancer: Linking Biology, Function, and Clinical Applications In Vivo Lake Buena Vista, FL January 23-27, 2002

Efficiency of Cutaneous Transfection and genetic immunization is greatly enhanced Using In Vivo Electroporation. Glasspool-Malone J, Drabick JJ, Somiari S, and Malone RW. Gene Therapy/Molecular Biology International Society Conference, Rhodes, Greece Aug. 24-31, 2000.

Optimized intradermal nucleic acid vaccination can protect Rhesus Macaques from Oral Challenge with Pathogenic SIVmac239. Rhodes GH, McChesney MB, Luciw PA, Torres JV, Gardner MB, Villinger F, Ansari AA, Lee K, Lena P, Smith J, Welsh J, Montbriand PM , Malone RW. Cutaneous Gene Therapy: Problems and Prospects. NIH, Bethesda, MD March 23-24, 2000.

Increased Efficiency of Cutaneous Transfection Using In Vivo Electroporation in Murine and Porcine Models. Somiari S, Drabick JJ, Glasspool-Malone J, King A, Walters R, Kesley KA and Malone RW. Cutaneous Gene Therapy: Problems and Prospects. NIH, Bethesda, MD March 23-24, 2000.

Marked Enhancement of skin Tissue Transfection by Aurintricarboxylic Acid in mice, rats, pigs and macaques. Glasspool-Malone J, Somiari S, Drabick JJ, Kesley KA and Malone RW. Cutaneous Gene Therapy: Problems and Prospects. NIH, Bethesda, MD March 23-24, 2000.

Airway eGFP expression in the non-human primate. McDonald RJ., Watts TL, Malone JG, and Malone RW. 13th Annual Cystic Fibrosis Foundation meeting. October 1999.

Developing dendritic cell polynucleotide vaccines for lentiviral disease. Jill G. Malone, Tammara L. Watts, Anne Hale and Robert W. Malone. American Society of Gene therapy meeting. Washington DC June 9-13, 1999.

Marked enhancement of direct respiratory tissue transfection by aurintricarboxylic acid. Jill Glasspool Malone and Robert W. Malone. American Society of Gene therapy Annual meeting. Washington DC June 9-13, 1999

DNA vaccination for SIV in Macaques P. Lena, P. Luciw, M. Gardner, R. Malone, J. Smith, M. McChesney, F. Villinger, and G. Rhodes "New Concepts in HIV Vaccine Development," NIH/AVRC NIH Bethesda 3-5 May 1999

Feline like dendritic cells-isolation, culture and genetic modifications using monocytic precursors. Glasspool-Malone JG, Watts TL, Malone RW. Symposium on Dendritic Cells in Fundamental and Clinical Immunology. Pittsburgh, PA. September, 24-28, 1998.

Zonula Occludens Toxin inhibits intracellular trafficking of luciferase DNA in susceptible cells. Watts TL, Malone JG, Malone RW, Fasano A. Molecular Mechanisms of Transcellular Signaling: Membrane Receptors to Transcription Factors. Advanced Study Institute Con. sponsored by NATO August, 1998

Self-limited gene expresson in-vitro in neuronal cell cultures and in-vivo in rat brain using mRNA/cationic liposomes. Hecker JG, Irion VR, Malone RW. Anesth Analgesia 94:S360, March 1997.

Escherichia coli B-glucuronidase and Photinus pyralis Luciferase reporter genes in an animal model. Ayar, S, Malone RW. AACC Molecular Pathology Conference. San Diego. November 21-23, 1997.

Langer DJ, Fisher KJ, Malone RW, Hecker JG, et. al.. Adenoviral Penton protein improves transfection efficiency of mRNA and plasmid DNA in primary neuroglial cells. Society for Neurosciences 26th Annual Meeting, Vol. 22(Part2):378.5. November 16-21. 1996

Structural and Functional Analysis of Cationic Transfection Lipids. Bennett MJ, Balasubramaniam RP, Aberle A, Malone JG, Nantz MH, Malone RW. 1995 N. American CF Conference, Dallas, Texas, October 12-15. Pediatric Pulmonology, (1995) S12 p220.

Genetic Immunization: Effects of Novel Priming and Boosting Strategies. Bassler KD, Montbriand, Bennett M, Malone JG, Nantz MH, Rhodes GH, Malone RW. 1995 N. American CF Conference, Dallas, Texas, October 12-15. Pediatric Pulmonology, (1995) S12 p278.

Development and testing of intramuscular and mucosal polynucleotide vaccines. Bassler, K.D., S.W. Barnett, P. Montbriand, B. Doe, J.G. Malone, G.H. Rhodes, M.J. Bennett, C. Walker, P.A. Luciw, K.S. Steimer, R.W. Malone (1995). 1995 International Symposium on Clinical Immunology, July20-23, 1995, San Francisco, California.

Mechanistic Studies and Cationic Lipid Structure/Function Analysis Leading to Improved Polynucleotide Transfection. Bennett MJ, Balasubramaniam RP, Bassler KD, Malone JG, Nantz MH, Malone RW. Journal of Investigative Medicine, V43 (S2) 330A.

A Novel Cationic Liposome Compound Class for use in Vivo and In Vitro Polynucleotide Delivery. Bennett MJ, Aberle AM, Balasubramaniam RP, Malone JG, Malone RW, Nantz MH. PolyGum (TfxTM Clinical Chemistry. (1994), 40(12)2341.

TfxTM Reagent, a Novel Cationic Lipid Formulation for Polynucleotide Transfection. Balasubramaniam RP, Oler JK, Groskreutz DJ, Goiffon V, Bennett MJ, Nantz, MH, Malone RW, Schenborn E. Clinical Chemistry. (1994), 40(12)2341.

Inhibition of Prostate Tumor Cell Growth Using Ribozymes Directed Against Interleukin-6. Erickson KL, Freedland SJ, Zadourian Z, Li J, Borchers H, Bennett M, Gumerlock , Larson T, Malone RW. Keystone Symposia on Molecular and Cellular Biology, Breckenridge, CO, January 15-21, 1995.

Polynucleotide Transfection as a Function of Cationic Lipid Structure. Quezada CM, Nantz MH, Malone RW. SACNAS Annual Conference, San Diego, CA, January 5-9, 1995.

PolyGum (Tfx), A Novel Cationic Liposome Compound Class for Use in vivo and in vitro Polynucleotide Delivery. Bennett MJ, Aberle AM, Balasubramaniam RP, Malone JG, Malone RW, Nantz MH. The Genetic Revolution: The San Diego Conference of the American Assoc. for Clinical Chemistry, Molecular Pathology Div., San Diego, CA, November 17-19, 1994.

Tfx, A Novel Cationic Lipid Reagent for Polynucleotide Transfection. The Genetic Revolution: Balasubramaniam RP, Oler J, Groskreutz D, Bennett MJ, Nantz MH, Malone RW, Schenborn E. The San Diego Conference of the American Association for Clinical Chemistry, Molecular Pathology Division, San Diego, CA, November 17-19, 1994.

Hepatic Gene Transfer Following Direct in vivo Injection. Malone RW, Hickmann MA, Walzem R, Bassiri M, Powell JS. Keystone Symposium on Molecular and Cellular Biology, Gene Therapy Conference, Keystone, CO, April 12-18, 1993.

Intrahepatic Reporter Gene Expression Following Direct in vivo Injection. Malone RW, Hickmann MA, Lehmann K, Walzem R and Powell JS. Cold Spring Harbor Laboratory Conference on Gene Therapy, Cold Spring Harbor, NY, September 22-26, 1992.

Successful Gene Transfection of Respiratory Epithelium in vitro Using Polyamine Containing Cationic Lipids. Robinson CB, Malone RW, Jessee J, Gebeyehu G, Wu R.ALA/ATS International Conference, San Francisco, CA, May 16-19, 1993.

Improvements in Cationic Liposome Vehicles for Human and Macaque Respiratory Epithelial Gene Delivery. Malone RW, Robinson C, Jessee J, Gebeyehu G, Isseroff R, Powell JS, Wu R. Sixth Annual North American CF Conference, October 15-18, 1992.

Gene Delivery with Cationic Liposomes Refractory to Serum Inhibition. Malone RW, Jessee J, Mack K, Wu R, Powell JS. American Federation for Clinical Research Annual Meeting, Clinical Research, Baltimore, MD, May 1992.

High levels of Messenger RNA Expression Following Cationic Liposome Mediated Transfection in Tissue Culture Cells. Malone R, Kumar R, Felgner P. NIH conference on "Self-cleaving RNA as a Anti-HIV Agent: Design and Delivery to Cells." Washington, DC, June 1989.

 

BOOK CHAPTERS:

  • Malone RW. "Present and Future Status of Gene Therapy.' Intro Chapter in Advanced Gene Delivery: From Concepts to Pharmaceutical Products. Editor: Allain Rolland. Harwood Academic Publishers (in press 12/98).
  • Malone RW. Toxicology of non-viral gene transfer. Editor, Walsh B. In: Non Viral Therapeutics: Advances, Challenges and Applications for Self-Assembling Systems. Boston: IBC's Biomedical Library Series. (1996) 4.1.1

 

CHAIRPERSON/ORAL PRESENTATIONS BY INVITATION:

Practical application of informatics technology in biomedical research. Malone RW at The BIO IT Coalition First Annual Conference. Titled: The Convergence of Bioscience and Information Technology: from Lab Bench to Marketplace. June 2002. (oral presentator/co-organizer).

Safe and efficient gene transfer using pulsed and electrical fields. Malone RW. The Mayo Clinic, The Molecular Medicine Program Seminar Series. May 16th 2002

Direct gene delivery: from serendipity to pulsed electric fields. Malone RW, American Society for Gene Therapy, 4th annual meeting. Seattle, WA. May 30-June 3, 2001. (oral presentation).

New Technologies and Emerging Opportunities in Gene Delivery and Functional Genomics. Malone RW, NIH, Bethesda, MD Nov 16-18, 2000. (oral presentation).

DNA electrotransfer into tissue: theory and applications. Malone RW, Gordon Conference on BIOELECTROCHEMISTRY MD at Queen's College Oxford, UK September 3-8, 2000. (oral presentation/section organizer).

In-vivo electroporation: Advances in Gene Delivery and genetic Vaccination. Malone RW Co-chairman and co-organizer of the International Gene Therapy Conference. Rhodes, Greece, August 24-31, 2000. (oral presentation/section organizer)

In-vivo electropermeabilization of murine and porcine skin enhances transfection efficiency and immunologic responses. Malone RW, American Society for Gene Therapy. Denver, CO. June 1-6, 2000. (oral presentation).

Increased Efficiency of Cutaneous Transfection Using In Vivo Electroporation in Murine and Porcine Models. Malone RW. Cutaneous Gene Therapy: Problems and Prospects. NIH, Bethesda, MD March 23-24, 2000. (oral presentation).

Pharmaceutical gene delivery: Past lessons predict clinical opportunities for Electroporation. Robert W. Malone, The Bioelectrochemical Society's XVth Symposium on Bioelectrochemistry and Bioenergetics. Strasburg, France. Sept. 25-27, 1999. (oral presentation).

"Aurintricarboxylic Acid (ATA) Strongly Enhances Direct Plasmid Transfection of Respiratory Tissues." Malone RW, Conference on Gene Therapy. Redwood City, CA, April 14-21, 1999. (oral presentation).

Chairman and speaker at the Gene Therapy/Molecular Biology International Society Conference. "Aurintricarboxylic Acid (ATA) Strongly Enhances Direct Plasmid Transfection of Respiratory Tissues." Malone RW, Conference on Gene Therapy. Crete, Greece, August 7-14, 1999. (chairperson/oral presentation).

"Polynucleotide Vaccines: From Direct Intramuscular DNA Injection to Dendritic Cells." Malone RW. Delivery of Proteins, Peptides and Genes. ECPI (European Centre for Pharmaceutical Information). Forte Posthouse Bloomsbury, London, December, 9-10, 1997 (chairperson/oral presentation).

"Dendritic Cell Polynucleotide Vaccination." Malone RW. New Approaches to Vaccine Development. NAVD 1997, Vienna, December 6-8, 1997 (chairperson/ oral presentation).

"Using Free Polynucleotides to Transfect Tissues and Express Proteins." Malone RW. Developing Self-Assembling Systems. International Business Communications, Annapolis, MD June 23-44, 1997 (scientific advisor/chairperson/ oral presentation).

"Comparative Studies of Materials and Dosing on Toxicity for Gene Delivery." Malone RW. ADDRESS: Cambridge Healthtech's Third Annual ARTIFICIAL SELF-ASSEMBLING SYSTEMS for GENE DELIVERY. San Diego, November 17-19, 1996. (scientific advisor/chairperson/ oral presentation).

"Alternative Systems for In Vivo Gene Delivery and Genetic Immunization." Malone RW. ADDRESS:, Rhodes GH, Hecker, JG. Institute of Human Virology 1996 Annual Meeting. Baltimore. September 7-13, 1996 (oral presentation).

"Toxicology of Non-viral Gene Transfer." Malone RW. Non-viral GENETIC THERAPEUTICS: Advances, Challenges and Applications for SELF-ASSEMBLING SYSTEMS. International Business Communications, Boston, MA June 6-7, 1996 (scientific advisor/chairperson/oral presentation).

"Mucosal Immune Responses Associated with Polynucleotide Vaccination." Malone RW. New Approaches to Bacterial Vaccine Development. Munich Germany, June 1996. (oral presentation).

"Structural and Functional Analysis of Cationic Transfection Lipids." Bennett MJ, Balasubramaniam RP, Aberle A, Crowe LM, Malone JG, Crowe JH, Nantz MH, Malone RW. Cambridge Healthtech Institutes Second Annual Artificial Self-Assembling Systems for Gene Transfer, Boston MA. September 28-29, 1995. (oral presentation).

"Genetic Immunization: Effects of Novel Priming and Boosting Strategies." Malone RW. New Approaches to Vaccine Development, NAVD 95, Vienna, Austria. April 14, 1995 (oral presentation).

"Genetic Immunization: Effects of Novel Priming and Boosting Strategies." Malone RW. Karolinska Institute, Stockholm, Sweden. April 18, 1995 (oral presentation).

"Direct in vivo Polynucleotide Transfer into Multiple Tissue Types." Malone RW. Gene Therapy and Nucleic Acid Vaccine Strategies, Rockville, MD, February 16-17, 1995. (oral presentation).

"Inhibition of Prostate Tumor Cell Growth Using Ribozymes Directed Against Interleukin-6." Freedland SJ, Zadourian Z, Li J-H, Borchers HM, Bennett MJ, Gumerlock PH, Larson T, Malone RW, Erickson KL. American Federation for Clinical Research, Monterey, CA, February 8-11, 1995. (oral presentation).

"A Model System to Assess Ribozyme Delivery to Target Cells." Freedland SJ, Zadourian Z, Bennett MJ, Balasubramaniam RP, Erickson KL, Malone RW. American Federation for Clinical Research, Monterey, CA, February 8-11, 1995. (oral presentation).

"Development and Testing of Intramuscular and Mucosal Genetic Vaccines." Bassler KD, Montbriand P, Torres JV, Malone JG, Luciw PA, Bennett M, Malone RW. American Federation for Clinical Research, Carmel, CA, February 8-11, 1995. (oral presentation).

"Ribozyme and mRNA Delivery Using Cationic Lipsomes." Malone RW. Keystone Symposia on Molecular and Cellular Biology, Breckenridge, CO, January 15-21, 1995. (oral presentation).

"Genetic Immunization of Mucosal and Parenteral Tissues." Malone RW. IBCs Second Annual International Conference on Mucosal Immunization, Genetic Approaches and Adjuvants, Rockville, MD, October 24-26, 1994. (oral presentation).

"Direct DNA Injection: A Method For in vivo Transfection Of Solid Tissues." Malone RW, Hickmann MA, Powell JS. Invited lecture at the Second Annual Conference of the National Cooperative Drug Discovery Groups for the Treatment of HIV Infection (NCVDG-HIV), Developmental Therapeutics Branch, DAIDS, NIAID, NIH, Washington, DC, July 11-16, 1993. (oral presentation).

"Regulation of Tumor Necrosis Factor-a Gene Expression Following Transfection: A Genetically Engineered Model for the Treatment of Toxic Shock." Erickson KL, Kisich KO, Malone RW, Feldstein PA, Powell JS. Keystone Symposium on Molecular and Cellular Biology, Genetically Targeted Research and Ther.: Antisense and Gene Therapy Conference, Keystone, CO, April 12-18, 1993. (oral presentation).

"Control of Macrophage TNF-a Gene Expression Following Intraperitoneal Ribozyme Transfection: A Genetic Therapy Model for the Investigation of Toxic Shock." Kisich KO, Malone RW, Feldstein PA, Jessee J, Gebeyehu G, Powell JS, Erickson K. Cold Spring Harbor Laboratory Conference on Gene Therapy, Cold Spring Harbor, NY, September 22-26, 1992. (oral presentation).

"Messenger RNA as a Drug: A Novel Approach for Expressing Foreign Proteins in Cultured Cells and in vivo." Malone RW. Northwestern University Medical School AOA Research Symposium, Chicago, IL, November 1989. (oral presentation).

"A Novel Approach to Study Packaging of Retroviral RNA by RNA Transfection." Malone R, Felgner P, Verma I. In RNA Tumor Viruses. Cold Spring Harbor Press, Cold Spring Harbor, NY, 1988. (oral presentation).